A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease

Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic throughout Latin America and is spread by worldwide migration. Diagnosis is currently limited to serological and molecular techniques having variations regarding their sensitivity and specificity. This work was aimed at developing a new sensitive, applicable, and cost-effective molecular diagnosis technique for loop-mediated isothermal amplification-based detection of T. cruzi (Tc-LAMP). The results led to determining a highly homologous satellite repeat region (231 bp) among parasite strains as a molecular marker for diagnosing the disease. Tc-LAMP was performed correctly for detecting parasite DNA (5 fg for the CL Brener strain and 50 fg for the DM28, TcVI, and TcI strains). Assay results proved negative for DNA from 16 helminth species and 7 protozoa, including Leishmania spp. Tc-LAMP based on the highly repeated T. cruzi satellite region is thus proposed as an important alternative for diagnosing T. cruzi infection, overcoming other methods' limitations such as their analytic capability, speed, and requiring specialized equipment or highly trained personnel. Tc-LAMP could be easily adapted for point-of-care testing in areas having limited resources

OEGMerge: un modelo de mezcla de ontologías basado en casuísticas

Desde hace tiempo la mezcla de ontologías es una actividad necesaria, sin embargo, los actuales métodos de mezcla de ontologías no tienen una casuística detallada ni una formalización precisa. Para la validación de estos métodos, es conveniente disponer de una casuística lo más completa posible. Por ello, en este artículo se presenta el modelo OEGMerge, desarrollado a partir de la experiencia del Grupo de Ingeniería Ontológica (OEG) de la UPM, en el que se describe detallada y formalmente la casuística de mezcla y las acciones a realizar en cada caso. En esta primera aproximación sólo se abarca taxonomía de conceptos, atributos y relacione

New Tendencies in Wind Energy Operation and Maintenance

[Abstract] Both the reduction in operating and maintenance (O&M) costs and improved reliability have become top priorities in wind turbine maintenance strategies. O&M costs typically account for 20% to 25% of the total levelized cost of electricity (LCOE) of current wind power systems. This paper provides a general review of the state of the art of research conducted on wind farm maintenance in recent years. It shows the new methods and techniques, focusing on trends and future challenges. In addition to this, this work includes a review of the following items: (i) operation and maintenance, (ii) failure rate, (iii) reliability, (iv) condition monitoring, (v) maintenance strategies, (vi) maintenance and life cycle and (vii) maintenance optimization As for offshore wind turbines, it is crucial to limit the maximum faults, since the maintenance of these wind farms is more complex both technically and logistically. Research into wind farm maintenance increased by 87% between 2007 and 2019, with more than 38,000 papers (Scopus) including “wind energy” as the main topic and some keywords related to O&M costs. The LCOE in onshore wind projects has decreased by 45%, while in offshore projects it has decreased by 28%. The O&M costs of onshore wind projects fell 52%, while in the case of offshore projects, they have declined 45%. Thus, the results obtained in this paper suggest that there is a change in research on wind farm operation and maintenance, as in recent years, scientific interest in failure has been increasing, while interest in the various techniques of wind farm maintenance and operation has been decreasing.This research was funded by the University of A Coruña (Spain) (Grant No. 64900)

Recent Advances in the Catalytic Enantioselective Reformatsky Reaction

This paper reviews the present state of the catalytic enantioselective Reformatsky reaction. Advancements in asymmetric versions of this reaction have recently led to a considerable extension of its scope and applicability, principally due to the use of highly active chiral ligands and very specific reaction conditions.M. A. F. I. is grateful for financial support from the Ministerio de Economía y Competitividad (MINECO) (CTQ 2012-35790) and the Consejería de Educación de la Comunidad de Madrid (programme AVANCAT, S2009/PPQ-1634). B. M. thanks the European Commission for a Marie Curie Integration Grant (FP7-PEOPLE-2012-CIG). I. M. P. and D. A. A. are grateful for financial support from the Spanish Ministerio de Ciencia e Innovación (MICINN) (project numbers CTQ2011-24165 and CTQ2010-20387, respectively) and from the University of Alicante

Lucene4IR: Developing information retrieval evaluation resources using Lucene

The workshop and hackathon on developing Information Retrieval Evaluation Resources using Lucene (L4IR) was held on the 8th and 9th of September, 2016 at the University of Strathclyde in Glasgow, UK and funded by the ESF Elias Network. The event featured three main elements: (i) a series of keynote and invited talks on industry, teaching and evaluation; (ii) planning, coding and hacking where a number of groups created modules and infrastructure to use Lucene to undertake TREC based evaluations; and (iii) a number of breakout groups discussing challenges, opportunities and problems in bridging the divide between academia and industry, and how we can use Lucene for teaching and learning Information Retrieval (IR). The event was composed of a mix and blend of academics, experts and students wanting to learn, share and create evaluation resources for the community. The hacking was intense and the discussions lively creating the basis of many useful tools but also raising numerous issues. It was clear that by adopting and contributing to most widely used and supported Open Source IR toolkit, there were many benefits for academics, students, researchers, developers and practitioners - providing a basis for stronger evaluation practices, increased reproducibility, more efficient knowledge transfer, greater collaboration between academia and industry, and shared teaching and training resources

ATR-FTIR as a potential tool for controlling high quality vinegar categories

Characterization of wine vinegars qualified with a Protected Designation of Origin (PDO) is crucial to certify their quality and authenticity. Spectroscopic techniques as Fourier transform mid infrared spectroscopy (FTIR) with Attenuated Total Reflectance (ATR) has been applied to investigate its potential as a rapid, cost-effective and non-destructive tool for characterizing different categories of high-quality vinegars. Spectra from 67 wine vinegars belonging to the PDOs “Vinagre de Jerez” and “Vinagre Condado de Huelva”, including their different established categories, were analyzed in the 4000–600 cm−1 infrared region. Changes associated to categories were observed in the region 1800–900 cm−1. These changes were assigned to certain compounds that increase during aging (e.g. acetic acids, alcohols, esters) or are characteristic of Pedro Ximenez category (e.g. sugars, furfural). Principal component analysis carried out on the most relevant spectral features, revealed that aging of vinegars clearly affect the ATR-FTIR spectra obtained in each PDO.Junta de Andalucía P12-AGR-160

Microwave-induced covalent functionalization of few-layer graphene with arynes under solvent-free conditions

A non-conventional modification of exfoliated few-layer graphene (FLG) with different arynes under microwave (MW) irradiation and solvent-free conditions is reported. The described approach allows reaching fast, efficient and mild covalent functionalization of FLG.M. P. is the recipient of the AXA Chair (2016-2023). This work was supported by the EU H2020-Adhoc-2014-20 Graphene Core1 (no. 696656)S

Podoplanin associates with CD44 to promote directional cell migration

This article is under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.-- et al.Podoplanin is a transmembrane glycoprotein up-regulated in different human tumors, especially those derived from squamous stratified epithelia (SCCs). Its expression in tumor cells is linked to increased cell migration and invasiveness; however, the mechanisms underlying this process remain poorly understood. Here we report that CD44, the major hyaluronan (HA) receptor, is a novel partner for podoplanin. Expression of the CD44 standard isoform (CD44s) is coordinately up-regulated together with that of podoplanin during progression to highly aggressive SCCs in a mouse skin model of carcinogenesis, and during epithelial-mesenchymal transition (EMT). In carcinoma cells, CD44 and podoplanin colocalize at cell surface protrusions. Moreover, CD44 recruitment promoted by HA-coated beads or cross-linking with a specific CD44 antibody induced corecruitment of podoplanin. Podoplanin-CD44s interaction was demonstrated both by coimmunoprecipitation experiments and, in vivo, by fluorescence resonance energy transfer/fluorescence lifetime imaging microscopy (FRET/FLIM), the later confirming its association on the plasma membrane of cells with a migratory phenotype. Importantly, we also show that podoplanin promotes directional persistence of motility in epithelial cells, a feature that requires CD44, and that both molecules cooperate to promote directional migration in SCC cells. Our results support a role for CD44-podoplanin interaction in driving tumor cell migration during malignancy.This work was supported by grant SAF2007-63821 from the Spanish Ministry of Science and Innovation (to M.Q.), the Royal Society University Research Fellowship (to M.P.), Medical Research Council (to G.E.J.) EU FP7 T3Net Consortium (GEJ), and Cancer Research UK (to G.E.J. and E.M.V.).Peer reviewe

Stress compensation by gap monolayers for stacked InAs/GaAs quantum dots solar cells

In this work we report the stacking of 10 and 50 InAs quantum dots layers using 2 monolayers of GaP for stress compensation and a stack period of 18 nm on GaAs (001) substrates. Very good structural and optical quality is found in both samples. Vertical alignment of the dots is observed by transmission electron microscopy suggesting the existence of residual stress around them. Photocurrent measurements show light absorption up to 1.2 μm in the nanostructures together with a reduction in the blue response of the device. As a result of the phosphorus incorporation in the barriers, a very high thermal activation energy (431 meV) has also been obtained for the quantum dot emission

Paradigma, ensayo y conclusión: La Casa de Bernard Rudofsky en tres actos

La Casa, —como así fuera bautizada— que Bernard Rudofsky construyó en la costa mediterránea española en 1971 es un estimable caso de estudio sobre las posibilidades operativas de la arquitectura popular en la práctica de la disciplina contemporánea. Es depositaria de referencias teóricas propias del mundo vernáculo, pues su autor, conocido por la exposición Arquitectura sin arquitectos, es emblema de este tipo de arquitectura al que dedicó gran parte de sus esfuerzos como teórico y polemista a lo largo de toda su vida. Además, se trata per se de un singular ensayo cabal de arquitectura, ya que supone una materialización práctica de su comprometida posición teórica. Y, por tanto, de este caso tal vez pueda extraerse alguna conclusión concreta que arroje luz sobre la posible operatividad o no de un tipo de arquitectura que, a pesar de ser habitualmente admirada y elogiada, presenta no pocos escollos para ser continuada en el contexto actual de la disciplina. El texto que se presenta, además, saca a la luz información inédita hallada en los diarios personales del arquitecto y que permite recomponer las circunstancias que rodearon la ideación y construcción de esta particular pieza de arquitectura.La Casa –or in English, the house– that Bernard Rudofsky built on the Spanish Mediterranean coast in 1971 is a valuable case study on the operational possibilities of popular architecture in the practice of this contemporary discipline. It is a repository of theoretical references typical of the vernacular world; its author, known for the exhibition Architecture without Architects, is emblematic of this type of architecture to which he dedicated much of his efforts as a theorist and polemicist throughout the course of his life. Additionally, La Casa is a unique architectural feat as it involves the practical materialization of its implicated theoretical position. It is possible that a concrete conclusion can be drawn from this case, which may shed light on the possible operability of a type of architecture that presents more than a few difficulties for the current context of the discipline, despite being habitually admired and praised. Additionally, the text presented here brings to light unpublished information found in the personal diaries of the architect that allows for the recreation of the circumstances surrounding the ideation and construction of this piece of architecture